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Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual Ryan Mehl

Contributor(s): Mehl, Ryan [author] | van Zee, Kari [author] | Kean, Kelsey [author] | Open Textbook Library [distributor]Material type: TextTextSeries: Open textbook libraryDistributor: Open Textbook Library Publisher: Oregon State University Description: 1 online resourceISBN: Subject(s): Chemistry -- TextbooksLOC classification: QD31.3Online resources: Access online version
Contents:
1. Background -- 2. Overview of Laboratory Responsibilities Tentative Schedule -- 3. Objectives for Week 1 -- 4. Concepts for Choosing and Expressing Mutants -- 5. Objectives for Week 2 -- 6. Concepts for Protein Expression -- 7. Methods for Protein Expression -- 8. Concepts for SDS-PAGE -- 9. Methods for SDS-PAGE -- 10. Objectives for Week 3 -- 11. Concepts for Protein Purification and Desalting -- 12. Methods for Protein Purification and Desalting -- 13. Microfluidizer Lysis Method -- 14. Objectives for Week 4 -- 15. Methods for Protein Standard Curves and SDS-PAGE -- 16. Objectives for Weeks 5-9 -- 17. Concepts for Enzyme Assays -- 18. Methods for Enzyme Assays
Subject: Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual
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1. Background -- 2. Overview of Laboratory Responsibilities Tentative Schedule -- 3. Objectives for Week 1 -- 4. Concepts for Choosing and Expressing Mutants -- 5. Objectives for Week 2 -- 6. Concepts for Protein Expression -- 7. Methods for Protein Expression -- 8. Concepts for SDS-PAGE -- 9. Methods for SDS-PAGE -- 10. Objectives for Week 3 -- 11. Concepts for Protein Purification and Desalting -- 12. Methods for Protein Purification and Desalting -- 13. Microfluidizer Lysis Method -- 14. Objectives for Week 4 -- 15. Methods for Protein Standard Curves and SDS-PAGE -- 16. Objectives for Weeks 5-9 -- 17. Concepts for Enzyme Assays -- 18. Methods for Enzyme Assays

Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual

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