000			01922nam a2200373 4500
001			OTLid0000854
003			MnU
005			20201105133411.0
006			m o d s
008			200627s2019 mnu o 0 0 eng d
020			_a
040			_aMnU _beng _cMnU
050		4	_aQD31.3
245	0	0	_aChemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual _cRyan Mehl
264		2	_bOpen Textbook Library
264		1	_bOregon State University
300			_a1 online resource
490	0		_aOpen textbook library.
505	0		_a1. Background -- 2. Overview of Laboratory Responsibilities Tentative Schedule -- 3. Objectives for Week 1 -- 4. Concepts for Choosing and Expressing Mutants -- 5. Objectives for Week 2 -- 6. Concepts for Protein Expression -- 7. Methods for Protein Expression -- 8. Concepts for SDS-PAGE -- 9. Methods for SDS-PAGE -- 10. Objectives for Week 3 -- 11. Concepts for Protein Purification and Desalting -- 12. Methods for Protein Purification and Desalting -- 13. Microfluidizer Lysis Method -- 14. Objectives for Week 4 -- 15. Methods for Protein Standard Curves and SDS-PAGE -- 16. Objectives for Weeks 5-9 -- 17. Concepts for Enzyme Assays -- 18. Methods for Enzyme Assays
520	0		_aChemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual
542	1		_fAttribution-NonCommercial
546			_aIn English.
588	0		_aDescription based on print resource
650		0	_aChemistry _vTextbooks
700	1		_aMehl, Ryan _eauthor
700	1		_avan Zee, Kari _eauthor
700	1		_aKean, Kelsey _eauthor
710	2		_aOpen Textbook Library _edistributor
856	4	0	_uhttps://open.umn.edu/opentextbooks/textbooks/854 _zAccess online version
999			_c20191 _d20191